An improved enzymatic pre-hydrolysis strategy for efficient bioconversion of industrial pulp and paper sludge waste to bioethanol using a semi-simultaneous saccharification and fermentation process

Abstract The current study investigated an integrated fermentation process for efficient conversion of pulp and paper sludge (PPS) material to bio-ethanol. To achieve maximum yield reducing sugar from the pretreated PPS, most influencing parameters such as enzyme dosages, surfactant dosages on PPS loadings were optimized in a batch saccharification using response surface methodology. experimental validation studies under optimal conditions 6% (w/w) solid loading condition, 0.16% concentration 158 FPU/gm resulted 45 ± 3.75% (w/w). Separate conducted with 4% 7% formulate best operating fed-batch 13% (w/w), 18% 22% high loading. maximal release glucose xylose 79.56 g/L 8.65 g/L respectively at 60 h. Further improvement bioethanol was established through adoption semi-simultaneous co-fermentation (S-SSCF) process. Fermentation co-cultivation two yeast species namely P. stipitis NCIM 3499 Baker’s ethanol 42.34 g/L 0.53 g/g Thus, demonstrated potential application waste feedstock production, which can be adopted by industries environment-friendly alternative common management.